Following on the success of the first Instruct Workshop on cryoEM best practices held in Harwell in May 2017 (link), NeCEN is organising the second version that will take place in Leiden, Netherlands on 15-16 Oct 2018.
The workshop is intended to promote sharing of experiences by EM user facilities and is open to EM facility scientists, manager and computing specialists both from Academia and Industry (max 30 participants). Presentations by some of the large cryoEM facilities will take place and some focus will be given towards operations, remote control, efficient usage of the machines and live processing in two separate sessions followed by round tables discussions.
On the second day, a tour of Thermo Fisher Nanoport and Factory in Eindhoven, where Krios microscopes are assembled, is organised.
Speakers:
Daniel Clare (eBIC, Harwell, UK) at Instruct Centre Diamond Light Source
Marta Carroni (SciLifeLab, Stockholm, Sweden)
Rebecca Thompson (Astbury Centre, Leeds, UK)
Wim Hagen (EMBL Heidelberg, Germany)
Pablo Conesa (CNB-CSIC, Madrid, Spain) at Instruct Centre for Computational Biology
Rodrigo Portugal (LNNano, Campinas, Brazil)
Ottilie von Loeffelholz (Integrated Structural Biology, IGBMC, Illkrich) at Instruct Centre France 1
Xueming Li (Tsinghua University, Beijing, China)
Dimitry Tegunov (MPI-BPC, Gottingen, Germany)
Christoph Mueller-Dieckmann (ESRF, Grenoble, France)
Registration to the workshop is now closed.
During the workshop, meals and coffees are covered. No support for accommodation or travel is provided. There is no onsite accommodation but several hotels nearby. We can assist you for booking a hotel if necessary.
Organisers
Ludo Renault, Susanne Roodhuyzen
Objectives
To share best practices between cryoEM centres for the delivery of services to academia and companies.
Programme
Day 1 – Monday 15th Oct
12:30 Registration – Light Lunch
13:30 Welcome and Introduction
Session 1: Operations and ensuring efficiency of access
13:45 An update on eBIC - Electron Bio-Imaging Centre (eBIC), Diamond Light Source, UK (Daniel Clare)
14:15 Swedish National CryoEM facility: combining screening and data collection for internal and external users - SciLifeLab, Stockholm, Sweden (Marta Carroni)
14:45 Cryo-EM at Leeds- approaches and challenges for high resolution data acquisition – Astbury Centre, Leeds, UK (Rebecca Thompson)
15:15 Unified EM setup: the benefits of SerialEM for facilities – EMBL, Heidelberg, Germany (Wim Hagen)
15:45 Coffee Break
16:15 – 17:15 Discussion 1 (chairs: Marta Carroni & Ludo Renault)
- Remote viewing
- Remote control
- Ensuring efficient usage of the machines
17:15 - 17:40 Short break and tour of NeCEN facility
17:40 - 18:20 Current status and perspectives of Cryo-EM in Brazil – LNNano, Campinas, Brazil (Rodrigo Portugal)
18:20 - 19:00 Cryo-EM at the European Synchrotron - Current status and future developments - European Synchrotron Radiation Facility, Grenoble, France (Christoph Mueller-Dieckmann)
19:30 Dinner
Day 2 – Tuesday 16th Oct
Session 2: Data management and live processing
9:00 Pipeline data collection at the IGBMC cryo-EM platform –IGBMC, Illkrich, France (Ottilie von Loeffelholz )
9:30 Scipion for facilities – CNB-CSIC, Madrid, Spain (Pablo Conesa)
10:00 System for high-throughput cryo-EM at atomic resolution - Tsinghua University, Beijing, China (Xueming Li)
10:30 Cryo-EM data pre-processing at full Warp – MPI-BPC Gottingen, Germany (Dimitry Tegunov)
11:00 Coffee Break
11:30 - 12:30 Discussion 2 (chairs: Pablo Conesa & Ludo Renault)
- Data Management
- Live processing
- Deliverables
12:30 Light Lunch
Optional tour of Thermo Fisher Scientific Nanoport and factory in Eindhoven
13:15 Departure for Thermo Fisher Scientific
15:30 Thermo Fisher factory and Nanoport visit
17:30 Returning to Leiden
19:30 end of meeting
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