2018 Cryo-EM School

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The Fall 2018 Cryo-EM School took place at the Netherlands Centre for Electron Nanoscopy (NeCEN). From the 22nd of October until the 16th of November, 6 participants received hands-on Titan Krios training from the experienced NeCEN EM scientists and Thermo Fisher applications support team. 

Part of the Cryo-EM School was a 3-day Image Processing Course organised in collaboration with the Biocomputing Unit of the Spanish National Center for Biotechnology (CNB).

Location

NeCEN, Leiden University, The Netherlands

Partners

  • Thermo Fisher Scientific
  • The Netherlands Centre for Electron Nanoscopy (NeCEN)
  • The Biocomputing Unit of the Spanish National Center for Biotechnology (CNB)

Curriculum

The curriculum is divided in two parts.

  1. Online lectures about the theory of cryo-EM single particle analysis as well as practical demonstrations by Grant Jensen (CalTech) and Matthijn Vos (Thermo Fisher Scientific) (http://em-learning.com). The school participants have to follow the online course and take the self-evaluation test before coming to NeCEN for practical hands-on training.
  2. Hands-on practicals on the different aspects taught online.
  • Cryo-EM sample freezing on a vitrobot
  • Cryo-EM grids clipping and loading for autoloader systems
  • Cryo-EM sample screening
  • Titan Krios alignments
  • Automated Single Particle Data Acquisition with EPU
  • Basics of image processing within Scipion Environment in the cloud

Participation

If you are interested in participating in following editions, please contact NeCEN directly: necen@biology.leidenuniv.nl

The course gives you 4 weeks access to 2 Krios microscopes equipped with Falcon 3EC and K2 Summit cameras, 2 dedicated expert teachers, housing with breakfast and lunch during the course days as well as transportation on site. Not included is travel to Leiden and dinners. The non-profit costs are ~30.000 euro or an equivalent of 30 days pre-purchased Thermo Fisher Scientific on-site application support.

For more details and specific questions, contact the head of NeCEN Ludo Renault: l.renault@biology.leidenuniv.nl